Ozonized glycerin is a highly stabilized solution that combines research-grade glycerin and ozone for various innovative applications. The solution can be formulated as a pure gel or diluted with saline or sterile water to specific concentrations. It is essentially a stabilized form of ozone.

Pure Gel: Undiluted ozonized glycerin.

Ozonated Glycerin Combined with Saline:

-2.5% (25,000 mcg/mL)

-5% (50,000 mcg/mL)

-10% (100,000 mcg/mL)

Ozonated Glycerin Combined with Sterile Water:

-2.5% (25,000 mcg/mL)

-5% (50,000 mcg/mL)

-10% (100,000 mcg/mL)

Materials and Equipment:

-Research-Grade Glycerin: A pure base ingredient with 99.5% or higher purity.

-Ozone Generator: Research-grade equipment capable of producing controlled concentrations of ozone gas (O₃).

-Ozone-Resistant Tubing: Made of silicone to withstand ozone’s oxidative properties.

-Bubbler System: For efficient infusion of ozone gas into the glycerin.

-Glass Reaction Vessel: Inert and ozone-resistant to prevent contamination.

-Cooling mechanism: The reaction vessel needs to be lodged in a cooling mechanism to prevent over heating

Procedure:

Preparation: Pour research-grade glycerin into a sterile glass reaction vessel. Set up the ozone generator and connect it to the bubbler system using ozone-resistant tubing.

Ozonation:

Turn on the ozone generator to produce ozone at a controlled concentration (e.g., 80 - 100 mg/L) at a flow rate of 1/16LPM or lower. Bubble ozone gas through the glycerin for a specified duration (up to seven days), depending on the desired ozonation level.

Dilution (if applicable):

For diluted solutions, mix the ozonized glycerin gel with saline or sterile water to achieve the desired concentration (2.5%, 5%, or 10%).

Stabilization:
Allow the ozonized glycerin to settle and stabilize.Perform quality control tests to ensure consistent ozonation levels.

Packaging:

Sterilized transfer of the ozonized glycerin into sterile, ozone-resistant glass vials. Seal with tamper-evident caps and label appropriately.

Ozonized glycerin should be stored at 2°C to 8°C (36°F to 46°F) in a refrigerator. It must be protected from light and heat to maintain stability. Freezing is not recommended, as it may alter the properties of the solution.

Ozonized glycerin is available in sterile, ozone-resistant glass vials in standard sizes, such as 5 mL, 10 mL, and 20 mL. Custom packaging options can also be provided upon request.

The terms are often used interchangeably, but both refer to glycerin infused with ozone. "Ozonized" is commonly used in technical contexts, while "ozonated" may be more casual.

Yes, customized concentrations and formulations of ozonized glycerin can be prepared to meet the unique needs of research projects.

While we are doing further testing, it appears ozonized glycerin has a half life of approximately 90 days when stored at 2°C to 8°C.