Ozonized Glycerin Explained: Chemical Properties, Analytical Challenges, and Research Prospects

-Unknown Chemical Composition: No conclusive analysis has determined the exact structure, and reputable chemists recommend deeper analysis.

-Unlikely to Be Glycerol Tri-Peroxide: Multiple experts have independently concluded that ozonized glycerin probably does not form the unstable tri-peroxide compound.

-Ozone Modifies Glycerin: When ozone is infused, it alters glycerin’s properties rather than simply retaining ozone gas, creating a distinct “new” substance.

-Complex & Costly Analysis: High-end, untargeted instrumentation—often exceeding $1,000,000—plus specialized expertise are needed to identify unknown structures.

-Differences from Ozone Gas: Claims of high ozone content (e.g., “300,000mcg per mL”) do not reflect the modified glycerin’s final chemical properties once ozone is infused.

Ozonized glycerin recently generated interest in the United States due to its potential applications in various industries. Despite this attention, the precise chemical composition of ozonized glycerin remains unknown. This overview clarifies current insights, addresses key questions about its molecular structure, and outlines the current objectives in understanding ozone infused glycerin.

Nobody knows exactly what ozonized glycerin contains from a chemical perspective, yet. This has nothing to do with its effectiveness. We have taken it upon ourselves to look deeper into this problem.

Some have speculated that glycerin altered by ozone is "glycerol tri-peroxide". Upon communication with three different world-class chemists, having worked at organizations such as Harvard University, Mayo Clinic, and Pfizer, they all independently agreed it is unlikely to be glycerol tri-peroxide. One of the chemist boasts 100 patents and near 600 publications. They are experts in every respect. These chemists provided potential chemical calculations but ultimately they all recommended running a proper analysis to determine the chemical composition, as this is the only way to have certainty. Furthermore, it is the only way to determine the abundance of chemicals within the ozonized glycerin (the quantity of each molecule).

Other individuals have provided speculations as to the molecules contained in ozonated glycerin. The reality is that it is still under investigation.

When ozone is infused into a substance, it changes the chemical properties of the substance. The substance does not retain ozone, even though ozone is used in the process.

As such, this is to be treated as something "new", even though the knowledge of its existence dates back to the 1890's. It is better to take this view than to perceive ozonized glycerin as an extension of ozone.

There is extremely little data on ozone-infused glycerin. To our knowledge, nobody has run a chemical analysis on the substance. Substances with "unknowns" are challenging. Analytical platforms (like LC–MS) rely on reference libraries of known compounds. When a substance doesn’t match any existing entries in those libraries, you cannot get immediate identification.

Instead, you need to determine molecular weights and generate potential molecular formulas, break the molecule into characteristic fragments, allowing you to piece together structural hints. We will likely need to patch together information from multiple platforms, costing well over $15,000, just for the analysis (which doesn't include the translation).

Identifying unknowns is like assembling a puzzle, each instrument provides a different “clue.” By compiling these clues and comparing them with known structural data, we can characterize the substance.

However, it’s time-intensive and requires specialized expertise.

In addition, you have to know exactly what you’re looking for to even identify the appropriate platforms and organizations that provide it. You cannot go to a standard “chemical analysis” company because they typically run limited, targeted assays (which is a simple search for common and already known compounds). The equipment used for untargeted analysis of unknown compounds often costs in excess of $1,000,000. Additionally, these are typically owned by large pharmaceutical companies (which don’t care about providing it as a service) or within academic institutions. Accessing these platforms within academic institutions is challenging unless you know how to do it.

We plan to perform an analysis and update this article in February.

To reiterate: nobody knows exactly what this is to our knowledge. And skepticism should be applied to those who claim they know what it is unless they can display the untargeted analysis they used.

Comparing ozone doses to ozonized glycerin is comparing apples and oranges - it is not the same. While a certain amount of ozone gas may be infused into the glycerin, that is not inherently representative of ozone gas. For example, saying there is 300,000mcg per mL of ozone infused into it can be technically accurate. That amount may have been infused into the glycerin. But it does not indicate the chemical properties of the glycerin itself, as the glycerin is modified by the ozone, not retaining the ozone molecule.